Activity and Prediction of the Safety Profile of Fatty

A fatty hydroxamic acid (FHA) mixture synthesized from olive oil triacylglycerides by 23 hydroxylaminolysis and composed predominantly of oleyl and linoleyl hydroxamic acid (OHA 24 and LHA, respectively) was characterized by means of IR, Raman, MS and 1D and 2D 1Hand 25 13C-NMR spectroscopy. The ratio of OHA and LHA (4:1) was confirmed by MALDI TOF/TOF 26 mass spectrometry. The radical scavenging and the Fe2+-chelating activity, as well as antioxidant 27 activity in β-carotene-linoleic acid and the Fe3+-reducing power assays of the FHAs yielded 28 positive results. The results of FHA cell toxicity on normal fibroblast (BJ) and a tumour cell line 29 (HeLa) revealed that the normal cell line was sensitive to FHAs within the entire range of applied 30 concentrations (5 × 10−4 mg/mL to 5 × 10−1 mg/mL), while the HeLa tumour cell line was sensitive 31 only at the highest FHA concentration (5 × 10−1 mg/mL). In silico target prediction indicated 32 cannabinoid receptors 1 and 2, the fatty-acid amide hydrolase 1 and hystone deacetylases as the 33 most probable targets of OHA and LHA. According to ADMET predictor analysis, the safety 34 profiles of OHA and LHA are comparable to that of SAHA (vorinostat) the histone deacetylase 35 inhibitor in use as an antineoplastic and immunomodulating agent. 36